A novel method of delivering a hydrophobic (water-insoluble) drug, in which the compound effectively acts as its own carrier, has shown comparable efficacy both in vitro and in vivo to the same drug formulated in a conventional delivery vehicle.
US researchers from the University of Buffalo (UB) and the Roswell Park Cancer Institute (RPCI) believe the technique, which could move swiftly into clinical trials, offers significant cost and toxicity advantages over the surfactant or other carrier vehicles commonly used to ensure the stable dispersion of hydrophobic agents into aqueous systems.
The new approach is based on a method known as reprecipitation, whereby a compound is dissolved in a mixable solvent such as DMSO and injected into water. The molecules then self-assemble as pure nanosized crystals and remain stably dispersed in water.
In an experiment described in the journal Molecular Pharmaceutics, the UB and RPCI researchers used reprecipitation to synthesise nanocrystals of a photosensitising anticancer drug, HPPH, which is currently in Phase I/II clinical trials at RPCI in Buffalo, New York.
They found the nanocrystal formulation was taken up by tumours in vitro and in vivo, showing similar efficacy to the same drug using conventional surfactant-based delivery (1% Tween-80/water).
The UB/RCPI team was originally investigating nanocrystals as a delivery method for hydrophobic dyes in bio-imaging applications, something they continue to pursue. While the use of reprecipitation to form nanocrystals of hydrophobic compounds is well established, as far as the researchers are aware there has been "no previous successful attempt to apply this technique in drug delivery, at least in the area of photodynamic therapy."
As they noted in Molecular Pharmaceutics, many commonly used pharmaceutical compounds are hydrophobic by nature. Delivering them to disease sites calls for special formulations using surfactants or other nanoparticle-based vehicles. The drawback is that these vehicles, or sometimes their by-products, have a tendency to increase the systemic toxicity of the drug formulation.
As nanocrystal formulations are a single-component system, they should not present any safety issues over and above those raised by the original drug, the researchers point out. This should also enable the technique to move into clinical trials "very fast, probably pending some additional approval by the Food and Drug Administration," they believe.
Moreover, the simplicity of the delivery method, with only DSMO and water required to formulate the nanocrystals, means the potential cost advantage over conventional delivery vehicles is "very high".
The UB/RCPI team is now conducting further in vivo studies with HPPH nanocrystals and looking at whether the technique can be used to deliver other hydrophobic drugs. Formation of the nanocrystals and their stability depends on the molecular structure of the drug, so only certain classes of compound will qualify, the researchers note.
"Right now," they add, "the nanocrystal technology looks most promising in the realm of photodynamic therapy of cancer, as both photosensitiser drugs and their nanocrystals have an inherent avidity for tumour sites."